<jats:title>Abstract</jats:title><jats:p>Two new cerebrosides, asperiamide B (<jats:bold>1</jats:bold>) and C (<jats:bold>2</jats:bold>), and two known aflatoxins, averufin and nidurufin, have been isolated from a Quanzhou marine fungus <jats:italic>Aspergillus niger</jats:italic> (MF‐16), and the structures were elucidated by spectroscopic data and chemical means. Among the compounds <jats:bold>5 </jats:bold>and<jats:bold> 6</jats:bold> showed moderate activities in inhibiting multiplication of the <jats:italic>Tobacco Mosaic Virus</jats:italic> (TMV).