<jats:title>Abstract</jats:title><jats:p>Two new triazoles, chrysotriazoles A and B (<jats:bold>1</jats:bold> and <jats:bold>2</jats:bold>, resp.), along with two known quinazolinones, <jats:bold>3</jats:bold> and <jats:bold>4</jats:bold>, two known 2‐(4‐hydroxyphenyl)acetamides, <jats:bold>5</jats:bold> and <jats:bold>6</jats:bold>, and two known <jats:italic>N</jats:italic>‐(4‐hydroxystyryl)formamides, <jats:bold>7</jats:bold> and <jats:bold>8</jats:bold>, were isolated and identified from the culture extract of <jats:italic>Penicillium chrysogenum</jats:italic> EN‐118, an endophytic fungus obtained from the marine brown alga <jats:italic>Sargassum pallidium.</jats:italic> The structures of the isolated compounds were elucidated by NMR‐spectroscopic analysis, and that of compound <jats:bold>1</jats:bold> was confirmed by X‐ray crystallographic analysis of its <jats:italic>p</jats:italic>‐bromobenzoate derivative. Compounds <jats:bold>4, 5</jats:bold>, and <jats:bold>7</jats:bold> showed moderate cytotoxicities against Du145, A‐549, and HeLa cell lines.