Two major constituents, retamycins E1 [1] and E2 [2], were isolated from the anthracyclinic complex retamycin. Both compounds are derivatives of y-rhodomycinone glycosidically linked to rhodosamine. In addition, 1 contains two molecules of rhodinose and 2 one of each rhodinose and deoxyfucose. Composition, sequence, and stereochemical details were determined by high field ${}^{1}H$ nmr spectroscopy and fabms. 1 and 2 were found to be identical with rhodilunancins $A$ and $B$ (= cosmomycins $A$ and $B$ ).