Isolation, Absolute Configuration, and Biological Activities of Chebulic Acid and Brevifolincarboxylic Acid Derivatives fromEuphorbia hirta

Journal of Natural Products
2020.0

Abstract

Twenty new chebulic acid and brevifolincarboxylic acid derivatives, including eight optically pure or achiral compounds (<b>1</b>-<b>7</b> and <b>14</b>) and six pairs of enantiomers (<b>8a</b>/<b>8b</b>-<b>13a</b>/<b>13b</b>), along with nine known analogues (<b>15</b>-<b>23</b>), were isolated from an EtOH extract of the aerial parts of <i>Euphorbia hirta</i>. The absolute configurations of the new compounds were assigned based on single-crystal X-ray diffraction analysis and comparison of the experimental and calculated ECD data. Racemic or scalemic mixtures of <b>8</b>-<b>13</b> were isolated, and their enantiomers were analyzed by chiral-phase HPLC-ECD measurements. Compound <b>12</b> possesses an unprecedented 2<i>H</i>-cyclopenta[<i>de</i>]chromene-2,5(4<i>H</i>)-dione scaffold. Compounds <b>12</b>, <b>20</b>, and <b>23</b> displayed moderate inhibitory effects against lipopolysaccharide-induced nitric oxide production in BV-2 microglial cells, while all the isolates exhibited significant DPPH radical scavenging activities with EC<sub>50</sub> values of 2.2-15.8 μM.

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