Aspergillus is a member of the phylum Ascomycota. There are over 185 known species of ascomycetes, about 20 of which are known to be harmful to humans and other animals. The Aspergillus species is known to produce more than 1000 secondary metabolites containing bioactive compounds, such as mevinolin, aflatoxin and citrinin.1 In a previous study, we reported that a fungus belonging to the Aspergillus species produces a novel secondary metabolite, JBIR-15.2 Therefore, in this study, we screened for novel secondary metabolites from cultures of Aspergillus species and succeeded in discovering novel furopyrrole compounds, JBIR-86 (1) and JBIR-87 (2), in addition to the known compounds, tensidols A and B (Figure 1a). In this study, we report the fermentation, isolation and structural determination of 1 and 2, and briefly describe their biological activities.