<jats:title>Abstract</jats:title><jats:p>Seven new metabolites, including three sesquiterpenoids, 10‐hydroxythujopsene (<jats:bold>1</jats:bold>), akotriol (<jats:bold>2</jats:bold>), and xylaritriol (<jats:bold>3</jats:bold>), one diterpenoid, cubentriol (<jats:bold>4</jats:bold>), one aliphatic derivative, akoenic acid (<jats:bold>5</jats:bold>), one alkaloid, akodionine (<jats:bold>6</jats:bold>), and one isocoumarin, akolitserin (<jats:bold>7</jats:bold>), together with seven known compounds, <jats:bold>8</jats:bold>–<jats:bold>14</jats:bold>, were isolated from the AcOEt‐soluble fraction of the fermentation broth of the endophytic fungus <jats:italic>Xylaria cubensis</jats:italic>, derived from the leaves of <jats:italic>Litsea akoensis</jats:italic> <jats:sc>Hayata</jats:sc> (Lauraceae). Their structures were elucidated by spectroscopic analyses, including 1D‐ and 2D‐NMR experiments, and by HR‐ESI‐MS mass spectrometry. Among the isolates, (−)‐(<jats:italic>R</jats:italic>)‐7‐hydroxymellein showed IL‐6 inhibitory activity with an <jats:italic>IC</jats:italic><jats:sub>50</jats:sub> value of 9.41 μ<jats:sc>M</jats:sc>.