<jats:title>Abstract</jats:title><jats:p>Two new glycosphingolipids, axiceramide A (<jats:bold>1a</jats:bold>) and B (<jats:bold>2a</jats:bold>), have been isolated as their peracetates (<jats:bold>1b</jats:bold> and <jats:bold>2b</jats:bold>, respectively) from the marine sponge <jats:italic>Axinella sp.</jats:italic>, and their structures elucidated by spectroscopic and chemical analysis. Axiceramide A and B, both composed of a complex mixture of homologues, are the first example of natural triglycosylceramides with three hexopyranose units all engaged in α‐glycosidic linkages.