A novel phenolic base, latifine (1), along with cherylline (2), was isolated from Crinum latifolium L. (Amaryllidaceae). Latifine has the molecular formula C17H19NO3, m.p. 215-217 °C, [α]D -27.9° (MeOH, c 0.32). Its planar structure was elucidated by spectral data (IR, 1H NMR, MS, 13C NMR) and methylation studies as 5-hydroxy-4-(4'-hydroxyphenyl)-6-methoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline. The S-configuration at C-4 was confirmed by the similarity of its optical rotatory dispersion (ORD) curve to that of cherylline (2) (both showing negative Cotton effects) and direct X-ray crystallographic analysis. Latifine is proposed to be a metabolite of N,O-dimethylnorbelladine (4) formed by ortho-coupling to the hydroxy group, whereas cherylline is formed by para-coupling to the hydroxy group of (4).