We have previously reported the isolation of N-demethylgalanthamine (1), lycorine (2), and crinamine (3) from Crinum asiaticum L. var japonicum BAKER (Amaryllidaceae). This paper deals with the isolation and structural elucidation of the alkaloids from two Crinum species of Amaryllidaceae, C. latifolium L. (Japanese name, Indohamayu), and C. bulbispermum MILNE-REDHEAD et SCHWEICKERDT (Japanese name, Afurikahamayu). A new base, 3-O-acetylhamayne (11), was isolated from Crinum latifolium L. (Amaryllidaceae) along with crinamine (3), powelline (4), crinine (5), 1-O-acetyllycorine (6), hamayne (8), undulatine (13), and cherylline (14). The bases 3, 4, 5, and 14, as well as diacetyllycorine (7), crinamidine (16), O-acetylcrinine (17), deacetylbowdensine (18), and bowdensine (19), were also isolated from Crinum bulbispermum MILNE-REDHEAD et SCHWEICKERDT (Amaryllidaceae).