<jats:p>From the latex of Euphorbia biglandulosa Desf. the cerebrosides 1-O-β-D-glucopyranosyl- 2-N-2′-hydroxy-cis-15-tetracosenoyl-4-hydroxy-eicosasphinga-as-10-enine (1), 1-O-β-D-glucopyranosyl- 2-N-2′-hydroxy-cis-17-hexacosenoyl-4-hydroxy-eicosasphinga-cis-10-enine (2) and 1-O-β-D-glucopyranosyl-2-N-2′-hydroxy-4-hydroxy-eicosasphinga-cis-10-enine (3) were isolated. Structures have been elucidated by <jats:sup>1</jats:sup>H NMR , <jats:sup>13</jats:sup>C NMR . MS spectroscopy, hydrolysis, and ozonolysis, too.