We previously isolated from the marine isolate of the fungus Aspergillus fumigatus KMM 4631 the five diketopiperazine antibiotics cyclotryprostatins A and B, fumitremorgin C, 12, 13-dihydrofumitremorgin C, and verruculogen [1]. Further research on metabolites of this strain afforded 1-5. Cultivation was performed under the previously described conditions [1]. The CHCl3:C2H5OH extract of A. fumigatus was chromatographed over a column of silica gel L (40/100 µm) with elution successively by CHCl3 and CHCl3:C2H5OH (10:1 and 5:1). Fractions eluted by CHCl3:C2H5OH (10:1) were combined, concentrated in vacuum to the minimal volume, and separated by HPLC over columns of Silasorb Si (ethylacetate:hexane, 4:1) and Diasphere-110-C18 (from 50 to 60% CH3OH in water) to afford 1 (4 mg), 2 (18 mg), 3 (3 mg), 4 (3.5 mg), and 5 (4 mg). Spectral data and two-dimensional spectra (DEPT, COSY-45, HSQC, HBMC, and NOESY) of 1 and 2 agree with those in the literature for fumiquinazolines C and D isolated from the marine isolate of A. fumigatus [2]. Spectral data for 3 agree with those in the literature for fumitremorgin B isolated from A. fumigatus parasitic on rice [3, 4]. Mass spectra and PMR spectra of 4 agree with those reported previously for bisdethiobis(methylthio)gliotoxin isolated from the fungi Gliocladium deliquescens [5], A. fumigatus [6], and Penicillium terlikowskii [7]. Mass spectra and PMR spectra of 5 agree with those reported previously in the literature for tryptoquivaline J isolated from the fungus A. fumigatus parasitic on rice [8]. Thus, A. fumigatus KMM 4631 is a new source of alkaloids of various structural types. Bisdethiobis(methylthio)gliotoxin (4) and tryptoquivaline J (5) were isolated from marine isolates of fungi for the first time.