The carotenoids from sea sponge Gelliodes callista were investigated. A new carotenoid gelliodesxanthin has been isolated and the structure 3-hydroxy-4-oxo-3′, 4′-didehydro-2′-apo-β, ψ-caroten-2′-al was proposed from the basis of the specCral data. Gelliodenone which seems to be an artifact carotenoid of gelliodesxanthin was also isolated and the structure was elucidated as 3-hydroxy-3′, 4′-didehydro-16′-methyl-17′-nor-β,ψ-carotene-4, 16′-dione. Besides gelliodesxanthin, β-carotene, spheroidenone, echinenone, canthaxanthin astaxanthin,β-doradexanthin, zeaxanthin, alloxanthin, fucoxanthin and idoxanthin were identified.