Studies on the constituents of Veratrum plants. I. Constituents of Veratrum maackii Reg.; isolation and structure determination of a new alkaloid, maackinine.

Abstract

The constituents of the roots and rhizoma of Veratrum maackii REG. (Liliaceae), which are used as a Chinese crude drug "Li-lu." were examined and a new steroidal alkaloid named maackinine was isolated along with five known alkaloids, germanitrine, verazine, angelovizvgadenine, zygadenine, and verazinin. The structure of maackinine was determined by the use of two-dimensional nuclear magnetic resonance (2-D NMR) techniques (fl-decoupled 1H-1H chemical shift correlation spectroscopy (COSY), 1H-1H COSY, 1H-13C COSY, and long-range 1H-13C COSY spectra). Assignments of 1H- and 13C-NMR signals of these steroidal alkaloids were also performed.