Two new steroidal alkaloids, 23-methoxycyclopamine 3-O-beta-D-glucopyranoside (1) and isoecliptalbine (2), were isolated from the root and rhizoma of Veratrum maackii REGEL, together with five known compounds, i.e., verussurine (3), verabenzoamine (4), verazine (5), isoverazine (6), and verazinine (7). Their structures were established by extensive analysis of spectroscopic data, as well as by comparison with literature data. Compounds 1-7 could cause DNAdamage in the cerebellum and cerebral cortex of mice in a dose-dependent manner by using single-cell gel electrophoresis (comet assay).