2-Ethyl-3-methylmaleimide N-β-d-glucopyranoside from the leaves of mangosteen (Garcinia mangostana)

Phytochemistry
1996.0

Abstract

From a methanolic extract of leaves of Garcinia mangostana, 2-ethyl-3-methylmaleimide N--D-glucopyranoside was isolated by adsorption chromatography on XAD resin followed by rotation locular countercurrent chromatography. After acetylation, separation was performed by column chromatography on silica gel and preparative HPLC on RP-18 phase. Identification was carried out by mass spectrometry (DCI, APCI) and nuclear magnetic resonance (1H NMR, 13C NMR, 1H 1H-COSY, HMQC). Assignment of the quateranry carbon resonances was achieved by HMBC experiments.

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