Two sulfur containing peptides have been isolated from a cationic peptide-rich fraction of dried Lentinus edodes by chromatographic technic. They have been identified as β-glutamylcystine and N,N-bis-γ-glutamylcystinylglycine based on chemical, chromatographic and spectroscopic properties. In the previous paper, the authors reported that dried Lentinus edodes (Berk) Sing. contained a large amount of oligopeptides and two main components were isolated and identified as lentinic acid and N,N'-bis-β-glutamylcystine. Further tests made on the cationic peptide-rich fraction of a mushroom extract showed the presence of some other unidentified sulfur containing peptides. The purpose of this paper is to report on the isolation and identification of two sulfur containing γ-glutamyl peptides, γ-glutamylcystine and N,N'-bis-γ-glutamylcystinylglycine, from the fraction.