<jats:title>Abstract</jats:title><jats:p>In the course of continuing search for the constituents of the root‐bark of <jats:italic>Hemandiu ovigera</jats:italic> L., led to the isolation of eight compounds: desoxypodophyllotoxin(I), thalicarpine(III)) dehydrothalicarpine(IV), ovigerine(V), hernangerine(VI), hernandonine(VIII) and two unknown substances, <jats:italic>mp</jats:italic>. 219–220° and <jats:italic>mp</jats:italic>. 269–270°. Desoxypodophyllotoxin( I) and thalicarpine(III) exhibited a distinctively cytotoxic activity against nasopharynx carcinoma was reported by S. M. Kupchan et.al<jats:sup>3,9</jats:sup>. Dehydrothalicarpine( IV) is merely a dehydro product of thalicarpine(III) at C–6a and C–7 of aporphine moiety. The isolation of dehydrothalicarpine(IV) made the first instance from <jats:italic>Hernandia ovigera</jats:italic> L. Comparison of their components distribution in each part of <jats:italic>Hernandia ovigera</jats:italic> was shown in Table I.