Determination of 5-methylcytosine content of four Cucurbitaceae species using high-performance liquid chromatography

Journal of Chromatography A
1991.0

Abstract

A rapid and sensitive procedure for the determination of methylcytosine in cucurbit DNAs using reversed-phase high-performance liquid chromatography is described. The results showed ca. 3.2-5.6% of methylcytosine in total DNA, i.e., about 2&30% of the cytosine residues appeared to be methylated. These results are discussed taking into consideration the influence of degree of methylation on the G + C determination of DNAs.

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