Determination of 4‐chloroindole‐3‐acetic acid methyl ester in Lathyrus, Vicia and Pisum by gas chromatography – mass spectrometry

Abstract

<jats:title>Abstract</jats:title><jats:p>4‐Chloroindole‐3‐acetic acid methyl ester was identified unequivocally in <jats:italic>Lathyrus latifolius</jats:italic> L., <jats:italic>Vicia faba</jats:italic> L. and <jats:italic>Pisum sativum</jats:italic> L. by thin layer chromatography, gas chromatography and mass spectrometry. The gas chromatographic system was able to separate underivatized chloroindole‐3‐acetic acid methyl ester isomers.<jats:p>The quantitative determination of 4‐chloroindole‐3‐acetic acid methyl ester in immature seeds of these three species was performed by gas chromatography – mass spectrometry using deuterium labelled 4‐chloro‐indole‐3‐acetic acid methyl ester as an internal standard. <jats:italic>P. sativum</jats:italic> contained approximately 25 mg kg<jats:sup>‐1</jats:sup>, <jats:italic>V. faba</jats:italic> 1–2 mg kg<jats:sup>‐1</jats:sup> and <jats:italic>L. latifolius</jats:italic> 2 mg kg<jats:sup>‐1</jats:sup> dry weight.