<jats:title>Abstract</jats:title><jats:p>Three new Lycopodium alkaloids, 2‐chlorohuperzine E (<jats:bold>1</jats:bold>), huperzines E′ (<jats:bold>2</jats:bold>), and F′ (<jats:bold>3</jats:bold>), along with two known compounds, huperzines E and F, were isolated from <jats:italic>Huperzia serrata</jats:italic> (<jats:sc>Thunb</jats:sc>.) <jats:sc>Trev</jats:sc>. Their structures were elucidated by spectroscopic methods.