<jats:title>Abstract</jats:title><jats:p>Two new lycopodine alkaloids, (12<jats:italic>β</jats:italic>)‐12‐hydroxyhuperzine G (<jats:bold>1</jats:bold>) and (5<jats:italic>β</jats:italic>,6<jats:italic>β</jats:italic>,15<jats:italic>α</jats:italic>)‐15‐methyllycopodane‐5,6‐diol (<jats:bold>2</jats:bold>), were isolated from the whole plants of <jats:italic>Huperzia serrata</jats:italic>, together with six known compounds, huperzines A, B, and G, phlegmariurine B, (8<jats:italic>β</jats:italic>)‐8‐hydroxyphlegmariurine B, and lycoposerramine D. Their structures were elucidated on the basis of spectroscopic analysis, including HR‐ESI‐MS, <jats:sup>1</jats:sup>H‐ and <jats:sup>13</jats:sup>C‐NMR, DEPT, <jats:sup>1</jats:sup>H,<jats:sup>1</jats:sup>H‐COSY, HSQC, HMBC, and NOESY data.