A new indole alkaloid, cigarindole A (1), together with five known indole alkaloids (2-6) were isolated from cigar tobacco. Their structures were elucidated using spectroscopic methods, including extensive 1D and 2D NMR techniques. Interestingly, compound 1 exhibited high antibacterial activity with bacteriostatic diameter within the range 20.6-26.7 mm against five pathogenic strains (Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, Bacillus subtilis, and Proteus spp). Compounds 2-6 exhibited obvious antibacterial activity with bacteriostatic diameter within the range 11.9-21.8 mm. In addition, compound 1 also the potential value for use as an antiseptic agent for cigarette tipping paper.