Two new pregnane alkaloids, (20S)-20alpha-cinnamoylamino-3beta-dimethylamino-5-en-pregnane (1) and (20S)-20alpha-cinnamoylamino-3beta-dimethylamino-pregnane (2), and four known alkaloids (+)-(20S)-20-(dimethylamino)-3-(3'R-isopropyl)-lactam-5alpha-pregn-2-en-4-one (3), axillaridine A (4), pachysamine M (5) and 20alpha-dimethylamino-16beta-hydroxy-3beta-senecioylamino-pregn-5-ene (6) were obtained from the whole herb of Pachysandra terminalis Sieb. et Zucc. Their structures were determined by various spectral techniques and computed electronic circular dichroism (ECD) data. Compounds 1-4 were tested for cytotoxicity against three human tumor cell lines and a human umbilical vein endothelial cell (HUVEC) line. Compound 4 exhibited moderate cytotoxicity against MCF-7, U251 and A549 cells with IC(50) values of 15.01 +/- 0.47 muM, 20.13 +/- 1.34 muM and 20.04 +/- 1.16 muM, respectively; compounds 1-3 showed weak cytotoxic activity against three tumor cells.