Dactylicapnos torulosa (D. torulosa) has been traditionally used as a therapeutic remedy. Here, a combined strategy using both phytochemical and biological approaches was conducted to discern the effective components of D. torulosa. Three new alkaloids, namely, (1-3), Torulosine A, 1-Methoxypseudoprotopine and 1,14-Dimethoxyprechilenine together with 33 (4-36) known compounds were isolated. The structures of the new compounds were fully established by extensive analysis of HR-ESI-MS NMR and CD spectroscopic data. These compounds were then screened using zebrafish bioassay methods for antithrombotic activities. Compounds 5 and 7 had the best antithrombotic effect with prevention rates of 100% at 50 mu M, and Compounds 8, 4, 9 and 11 at 50 mu M had prevention rates of 81.3%, 79.4%, 84.4% and 79.3%, respectively. The antithrombotic effect of compounds may be related to their inhibitory effect on platelet aggregation. The present study contributes to the diverse chemical and bioactivity data of the Dactylicapnos genus.