India is considered as the potential feedstock and richest sources of herbals that have potent medicinal values. The wide distribution of flora in India offers medicinally active plants and their derivatives for treatment of various ailments like heart disease, skin disorders and various cancers. Jasminium pubescens (J. pubescens) (Oleaceae) is an ornamental sub-shrub that contains various bioactive remedies that are exploited for various disorders. The objective of present study was focused on the phytochemical screening of Jasmine plant leaves for the presence of flavonoids, alkaloids, tannins, coumarins, terpenoids, proteins and amino acids in aqueous, methanolic and petroleum ether extracts. The antioxidant activity was determined spectrophotometry using DPPH and FRAP method and presence of total phenol and flavonoids were also determined. The agar well diffusion method was employed for antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results of study indicated that the methanolic extracts of Jasmine leaves showed significant antimicrobial activity against S. aureus (25.7±0.84mm), minimum inhibitory concentration (12.5mg/ml) as compared to other extracts and Ofloxacin as a standard. The methanolic, petroleum ether and aqueous leaf extracts of J. pubescens are utilized for production of newer bioactive phytometabolites having potent antimicrobial and antioxidant activity for curing various ailments. © RJPT All right reserved.