Zygophyllum coccineum is a halophytic plant species that belongs to the Zygophyllaceae family and can survive in high-salted and coastal-land areas. The present study intends to identify Z. coccineum extract. GC/MS spectral analysis was used to characterize the volatile components of the plant extract and estimate their biological profiles. From the methanol extract of Z. coccineum, six components were discovered by GC-MS analysis, with 2-ethylhexanoic acid is the major volatile component with (82.98%) of total composition. It was found that 91b,5,5,6a-tetramethyl-octahydro-1-ox a-cyclopropane[a]inden-6-one had a high composition percentage (8.13%) in the terpene category, and 6H-[1]benzopyrano[4,3-C]isoquinoline-6,11(5H)-dione was found by composition percentage (2.90%) from the category of alkaloid. When evaluated for DPPH antioxidant activity, at 1000 mg/ml, the scavenging activities of 61.89 for Z. coccineum extract. However, the samples with the lowest concentration (100 mg/ml) demonstrate the weakest antioxidant activity overall. With IC50 values of 5.24, 8.80, and >100 g ml-1 for HepG-2, PC3, and WI-38, respectively, the 50% methanol extract of Z. coccineum demonstrated an enhanced potential anticancer impact and significant cytotoxicity for the two tumor cells. As a result, the biological activity of Z. coccineum supports its use as a traditional therapy for a variety of diseases, including cancer treatment. ©2024 National Information and Documentation Center (NIDOC)