Qinggusan is the 69th prescription in the first batch of "Catalogue of Ancient Chinese Classic Formulas". In modern clinical practice锛?Qinggusan is mainly used to treat noninfectious fever. However锛?because few studies on Qinggusan reference samples and their quality value transfer are available锛?the development and promotion of its compound preparations are restricted. Therefore锛?establishing an accurate and comprehensive quality control method to clarify the critical quality attributes of Qinggusan reference samples is of great importance. In this study锛?15 batches of Qinggusan reference samples were processed to determine the range of their dry extract ratios. Quantitative high-performance liquid chromatography 锛圚PLC锛?fingerprint analysis was performed using a Waters Symmetry Shield RP18 column 锛?50 mmx4.6 mm锛?5 mum锛?with acetonitrile-0.1% 锛坴/v锛?formic acid aqueous solution as the mobile phase in gradient elution mode. The flow rate was 1.0 mL/min锛?the column temperature was 30 鈩冿紝 and the detection wavelength was 254 nm. The HPLC fingerprints of the Qinggusan reference samples were established under these conditions to evaluate their similarity. The established method was systematically validated and found to demonstrate good precision锛?repeatability锛?and sample stability. Subsequently锛?characteristic peaks were identified and attributed by HPLC-quadrupole-time-of-flight-mass spectrometry 锛圚PLC-Q-TOF-MS锛?analysis. MS was performed in electrospray ionization mode锛?the data were collected in both positive- and negative-ion modes锛?and the detection range was m/z 50-2000. The contents and transfer rate ranges of the index components锛?namely锛?gentiopicrin锛?mangiferin锛?picroside 鈪★紝 picroside 鈪狅紝 and glycyrrhizic acid锛?were determined to analyze the quality value transfer of the samples. The results demonstrated that the dry extract rate of the 15 batches of Qinggusan reference samples ranged from 24.10% to 26.88% and that their fingerprint similarities were generally greater than 0.95. Twelve common peaks were identified by reference identification锛?literature comparison锛?and high-resolution MS analysis. Twelve compounds锛?including six iridoid glycosides锛?two flavonoids锛?one alkaloid锛?one triterpenoid saponin锛?and two others. Among them锛?L-picein锛?androsin锛?picroside 鈪ｏ紝 picroside 鈪?and picroside 鈪?were from Picrorhizae Rhizoma锛?loganin acid锛?swertiamarin and gentiopicrin were from Gentianae Macrophyllae Radix锛?neomangiferin and mangiferin were from Anemarrhenae Rhizoma锛?dichotomine B was from Stellariae Radix锛?and glycyrrhizic acid was from Glycyrrhizae Radix et Rhizoma. The five key components presented good linear relationships in their respective linear ranges锛?and all correlation coefficients were higher than 0.999. The relative standard deviations 锛圧SDs锛?of precision锛?stability锛?and repeatability were less than 1.3%. The average recoveries varied between 95.92% and 102.5%锛?with RSDs less than 3.9%锛?these values meet the requirements defined in the 2020 edition of the Chinese Pharmacopoeia. The contents of gentiopicrin锛?mangiferin锛?picroside 鈪★紝 picroside 鈪狅紝 and glycyrrhizic acid in the 15 batches of reference samples were in the range of 17.92-27.55锛?1.83-4.42锛?23.08-36.44锛?8.43-15.04锛?and 0.94-2.39 mg/g锛?respectively锛?and their transfer rates from the decoction pieces to the reference samples were 47.91%-63.95%锛?22.96%-59.39%锛?60.82%-77.82%锛?64.25%-99.53%锛?and 15.30%-39.30%锛?respectively. In this study锛?the chemical components of Qinggusan reference samples were comprehensively identified and their quality value transfer was studied through the combination of HPLC fingerprinting and MS. Clarification of the critical quality attributes of Qinggusan reference samples could provide a basis for the quality control of Qinggusan compound preparations.