A new aconitine-type diterpenoid alkaloid, villosutine (1), was isolated from the whole plant of Aconitum franchetii var. villosulum, together with four other known aconitine-type diterpenoid alkaloids, one known pyro-type diterpenoid alkaloid and five known franchetine-type diterpenoid alkaloids. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HR-ESIMS, 1D and 2D NMR experiments. © 2016 Chinese Chemical Society & SIOC, CAS.