One new pyrrolyl 4-quinolinone alkaloid, penicinoline E (1), together with three known deriverites, methyl-penicinoline (2), penicinoline (3), and quinolactacide (4), were isolated from the marine-derived fungus Penicillium sp. ghq208. The structures of these isolated compounds were elucidated by spectroscopic methods. Compounds (2, 3) exhibited moderate cytotoxicities against the HepG2 cell line with IC50 values of 11.3 and 13.2 mu m, respectively.