Phytochemical investigation on Fritillaria pallidiflora led to the isolations of one new steroidal alkaloid, (20R,22R,23R,25R)-3β,23-dihydroxy-N-methyl-veratram-13(17)-en-6-one (14) and fifteen known compounds: five steroidal saponins (1-5), three phenolic glucosides (6-8), three nucleoside compounds (9-11), one amino acid (12), four steroidal alkaloids (13-16). Compounds 12, 14 were isolated from F. pallidiflora for the first time. Compounds 1-6 were isolated from the genus Fritillaria for the first time and compounds 7-8 were isolated from the family Liliaceae for the first time. The chemotaxonomic significance of these compounds was summarized. © 2012 Elsevier Ltd.