A new steroidal alkaloid, hupehenizioiside (1), together with four known steroidal alkaloids hupehenizine (2), hupehenirine (3), peiminine (4) and hupeheninoside (5), were isolated and identified from the bulbs of Fritillaria lichuanensis . The structure of hupehenizioiside (1) was determined to be (20 R ,25S )-5 alpha,14 alpha,17 beta-cevanine-6-oxo-3 beta-O-beta-D-glucoside by spectral analysis and chemical evidence. Compounds (2)-(5) were isolated from Fritillaria lichuanensis for the first time.