Two new norfumiquinazolines, cottoquinazolines E and F (1 and 2, resp.), together with pyripyropene A (3), were isolated from the fungus Neosartorya fischeri NRRL 181. The structures of the new isolates were established by spectroscopic methods, including UV, IR, HR-ESI-MS, and extensive 1D- and 2D-NMR techniques. To the best of our knowledge, this is the first report on norfumiquinazolines and pyripyropenes produced by the genus Neosartorya.