Two new sesquiterpene pyridine alkaloids hypoglaunines E (1) and F (2) and a new triterpenoid saponin hypoglaside A (3), together with a known diterpenoid glucoside 11-O-d-glucopyranosyl neotriptophenolide (4) and two known triterpenoids 23-nor-oxopristimerol (5) and 2,3-dihydroxy-6-oxo-D:A-froedo-24 nor-1,3,5(10),7-oleanatetraen-29-oic acid (6), have been isolated from the root barks of Tripterygium hypoglaucum. Their structures were elucidated by NMR spectroscopy and MS analysis. Compounds 1-3 were screened for their cytotoxic activities against five cancer cell lines, but all of them were inactive. © 2012 Copyright Taylor and Francis Group, LLC.