An aporphine alkaloid fromNelumbo nuciferaas an acetylcholinesterase inhibitor and the primary investigation for structure–activity correlations

Natural Product Research
2012.0

Abstract

N-methylasimilobine (1), a new-found strong acetylcholinesterase (AChE) inhibitor, along with two weakly active aporphine alkaloids, nuciferine (2) and nornuciferine (3) were separated from Nelumbo nucifera. N-methylasimilobine (1) inhibited 50% of AChE activity at the concentrations of 1.5 +/- 0.2 microg mL(-1) when the standard IC(50) value of Physostigmine was 0.013 +/- 0.002 microg mL(-1). The mode of AChE inhibition by 1 was reversible and non-competitive. In addition, molecular modelling was performed to explore the binding mode of inhibitor 1 at the active site of AChE.

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