Curvulamine is a novel alkaloid with strong antimicrobial activity produced by the marine-derived fungus Curvularia sp. IFB-Z10. In this work, the effects of different carbon and nitrogen source on curvulamine production were firstly investigated. Different morphologies were formed and pellet-like growth of fungi was found positive for the curvulamine formation, and sucrose, yeast extract and sodium nitrate revealed most suitable for curvulamine formation. Subsequently, four seed culture methods were employed to examine the linkage between pellet shape and curvulamine production. The mean pellet diameter was significantly decreased and the production was improved to 17.97 mg/L by method M3, which used baffled shake flask and glass homogenizer to prepare the homogenized seed broth. Furthermore, microparticle-based morphology engineering was used to control the pellet shape and enhanced the curvulamine production. By supplementation of talc powder (200 mesh, 5 g/L), the mean pellet diameter dramatically decreased and the fermentation titer reached 33.05 mg/L, which was about 1.9-fold of the control (17.21 mg/L). In addition, the fermentation process was scaled up in a 5-L bioreactor and the curvulamine production reached 21.63 mg/L, which was about 3.3-fold of the control (6.58 mg/L). The results obtained provided a new approach to enhance curvulamine production. © 2015 Published by Elsevier Ltd.