During a reexamination of the alkaloidal constituents of Tylophora indica, three new alkaloids (designated A, B, and C) were isolated. Analytical and spectral data indicate that these alkaloids are related to tylophorine and tylophorinine, which are also present in the plant. Alkaloid B is shown to be a desmethyl‐tylophorine, and Alkaloid C is shown to be a desmethyltylophorinine. T. dalzellii has a relatively low alkaloid content, and desmethyl‐tylophorinine is the major constituent. A brief description of the antileukemic activity of desmethyltylophorinine is presented. Copyright © 1971 Wiley‐Liss, Inc., A Wiley Company