An NMR study of a new stereoisomer of isoreserpiline pseudoindoxyl alkaloid is described. The complete 1H and 13C NMR assignments of the compound were carried out using COSY, HMQC and HMBC. Stereochemistry at carbons C-2, C-3, C-15, C-20 and C-19 was established using values of 1H chemical shifts, coupling constants and NOESY experiments. © 2003 John Wiley & Sons, Ltd.