A new benzopyrroloisoquinoline alkaloid, fistulosine (1), was isolated from the stem-bark of Ficus fistulosa (Moraceae) collected in Singapore, along with three known phenanthroindolizidine alkaloids, (-)-13a alpha-antofine (2),(-)-14 beta-hydroxyantofine (3) and (-)-13a alpha-secoantofine (4).(-)-13a alpha-Antofine (2) accounted for the antifungal activity against Aspergillus fumigatus and Candida albicans originally observed in the crude alkaloid extract. (C) 2009 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.