Three new steroidal alkaloids, named sarcovagenine A-C, were isolated from roots of Sarcococca vagans. Their structures were established on the basis of spectral evidence and biogenetic considerations as (20S)-20- (dimethylamino)-3β-(tigloylamino)-5α-pregn-16-en-2β,4β-diol, (20S)-4β- acetoxy-20-(dimethylamino)-3β-(tigloylamino)-5α-pregn-16-en-2α-ol, and (20S)-20-(dimethylamino)-3-(tigloylamino)-5α-pregn-2,16-dien-4-one, respectively.