From the bark of Aspidosperma chakensis Spegazzini there have been isolated quebrachamine and a new alkaloid, now named spegazzinine (C21-22H28-30N2O3). The presence of a N-acetyldihydroindole nucleus, a phenolic and an alcoholic hydroxyl group has been established and evidence for the location of the phenolic substituent is presented. Spegazzinine may be a hydroxylated demethylaspidospermine. © 1956, American Chemical Society. All rights reserved.