Stationary cultivation of Claviceps purpurea D-3-18 on a liquid medium enriched with D,L-isoleucine yielded sclerotial mycelium containing, apart from α-and β-ergokryptine (1,2) and ergocornine, three new alkaloids: a 5'-epimer of β-ergokryptine (3), β-ergokryptam (6), and a lactam alkaloid from a new group of ergopeptam alkaloids containing Lisoleucine as the first amino acid, i.e., N{N(d-lysergyl)-L-isoleucyl]-L-isoleucyl-D-proline lactam (5). For this substance the name β,β-ergoannam is proposed. © 1984, American Chemical Society. All rights reserved.