Two new lupine alkaloids, (−)-l3αtigloyloxymultiflorine [2] and (+)-(trans-(d+)′-acetoxycinnamoyl)epilupinine [10], were isolated from 7- to 10-day-old seedlings of Lupinus hirsutus (blue lupine) together with ten known alkaloids. The structures of the new alkaloids were confirmed by spectroscopic methods and by chemical transformations. We also clarified variations of the alkaloidal content at various stages of seedling growth. © 1991, American Chemical Society. All rights reserved.