A new lupin alkaloid, (-)-13 beta-hydroxymultiflorine, was isolated from an ethanol extract of the seeds of Lupinus varius, together with 13 known lupin alkaloids. In addition, two new lupin alkaloids, (+)-2 beta-hydroxyaphylline and (+)-13 alpha-hydroxyaphyllidine, were isolated from seeds of L. hartwegii, together with another 11 known lupin alkaloids. The identification of all compounds was established by spectral analysis. (C) 1997 Elsevier Science Ltd.