The isolation and structure elucidation of a new ergot peptide alkaloid, ergobine [1], from submerged cultures of Claviceps purpurea strain 231 FI, are described. Its purification and identification were achieved by feeding experiments with a labelled precursor. The structural details have been established through chemical degradation, uv spectroscopy, hplc, amino acid analyses, 1H nmr, eims, fdms, and its isomerization to ergobinine. © 1993, American Chemical Society. All rights reserved.