A new bisbenzylisoquinoline alkaloid, (+)-limacusine-2'-beta-N-oxide [1], was isolated from the leaves of Anisocycla jollyana, along with nine known alkaloids: (-)-2'-norlimacine, (-)-2-norlimacine, (-)-limacine-2'-beta-N-oxide, (+)-homoaromaline, (+)-trilobine, (+)-isotrilobine, (+)-dehydro-1,2-telobine, and (+)-remrefidine. The structure of 1 was established by spectral methods.