Jineol (1), a new quinoline alkaloid, was isolated from the centipede Scolopendra subspinipes, and its structure was elucidated by 2D NMR experiments. Jineol exhibited modest cytotoxic activity in vitro against the growth of human tumor cell lines: A-549, SKOV-3, SK-Mel-2, XF-498, and HCT- 15 cells.