Phytochemical investigations of the stems of Tinospora crispa led to the isolation of one new aporphine alkaloid, N-formylasimilobine 2-O-β-D-glucopyranoside (1), along with six known alkaloids, N-formylasimilobine 2-O-β-D-glucopyranosyl-(1→2)-β-D- glucopyranoside (2) (tinoscorside A), N-formylanonaine (3), N- formyldehydroanonaine (4), N-formylnornuciferine (5), N-demethyl-N- formyldehydronornuciferine (6), magnoflorine (7), paprazine (8), N-transferuloyltyramine (9), and cytidine (10). The structure of new compound 1 was deduced on the basis of spectroscopic data.