Aim: To investigate the chemical constituents of Yunnan local medicinal plants Alstonia scholaris. Methods: Silica gel column chromatography was used to isolate the constituents, and spectroscopic techniques (NMR, IR, UV and MS) were used for structural elucidation. Results: Four picrinine-type monoterpenoid indole alkaloids, 5β-methoxyaspidophylline (1), picrinine (2), picralinal (3) and 5-methoxystrictamine (4) were obtained from the leaves of Alstonia scholaris. Conclusion: Compound 1 is a new monoterpenoid indole alkaloid.