Cyclohexane-fused octahydroquinolizine (COHQ) alkaloids present an unwonted carbon framework among alkaloids isolated from genus Myrioneuron, which featured with bridge-ring and hemiacetal groups. Continued chemical investigation of Myrioneuron faberi led to the isolation of four COHQ related structures, β-myrifabral C (1), α-myrifabral C (2), β-myrifabral D (3), and α-myrifabral D (4). 1 and 2 were inseparable hemiacetal epimers (cluster A), as did 3 and 4 (cluster B). The structures of 1–4 were elucidated on basis of MS and NMR spectra. In vitro, cluster A showed moderate inhibition activity against hepatitis C virus (HCV) replication with therapeutic index (CC50/EC50) of 74.0. © 2016 Elsevier Ltd