A series of 1,3,5-triazine derivatives that contain aniline, coumarins (4-hydroxy coumarin and 7-hydroxy-4 methyl coumarin) and different piperazine moieties as substituent on the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. Comparative studies were performed on above series, which were synthesized with conventional and microwave heating methods. The microwave method was observed to be more beneficial as it provides an increase in yield and 90–95% reduction time. All the synthesized compounds were then examined for their efficacy against two Gram -ve bacteria (Escherichia coli and Pseudomonas aeruginosa), two Gram ?ve bacteria (Staphylococcus aureus and Bacillus subtilis) and two fungal species (Candida albicans and Aspergillus niger) with an intent to overcome multiple drug resistance to the pathogenic strains. All the synthesized compounds were structurally elucidated by IR, 1 H NMR, 13C NMR and elemental analysis.